Premium
Effects of hypothalamic peptides on electrical activity and membrane currents of ‘patch perforated’ clamped GH 3 anterior pituitary cells
Author(s) -
Barros Francisco,
Delgado Luis M.,
Maciá Carmen,
de la Peña Pilar
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80243-v
Subject(s) - somatostatin , hyperpolarization (physics) , chemistry , patch clamp , endocrinology , electrophysiology , anterior pituitary , medicine , membrane potential , calcium , biophysics , voltage clamp , potassium , hormone , biology , biochemistry , organic chemistry , nuclear magnetic resonance spectroscopy
‘Perforated‐patch’ recordings of rat anterior pituitary GH, cells allow long and stable monitoring of electrical activity and membrane currents. Under current clamp conditions, the biphasic effect of thryotropin releasing hormone (TRH) consisting of a transient hyperpolarization followed by a longer phase of increased action potential frequency is fully preserved. Somatostatin suppresses action potential activity and antagonizes the second phase of enhanced spiking caused by TRH. Voltage clamp records of isolated currents indicate that TRH affects calcim‐dependent potassium currents, but does not alter either voltage‐dependent potassium or calcium currents at times and concentrations at which the electrical activity is increased.