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Induction of programmed cell death (apoptosis) in mature lymphocytes
Author(s) -
Lucas Miguel,
Solano Francisca,
Sanz Aureo
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80239-y
Subject(s) - apoptosis , programmed cell death , microbiology and biotechnology , protein kinase c , cell culture , phorbol , chemistry , biology , fetal bovine serum , kinase , cell , immunology , biochemistry , genetics
The apoptosis of human peripheral blood lymphocytes was analyzed by the breakdown of DNA into oligonucleosome‐sized fragments. The mature lymphocytes were rendered sensitive to apoptosis by either the omission of fetal bovine serum in the culture medium or the addition of polymyxin B. In the first case it was counteracted by phorbol myristate acetate. The possible involvement of protein kinase C in cell survival is pointed out.