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Evidence for gene duplication forming similar binding folds for NAD(P)H and FAD in pyridine nucleotide‐dependent flavoenzymes
Author(s) -
McKie James H.,
Douglas Kenneth T.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80236-v
Subject(s) - nad+ kinase , biochemistry , reductase , dehydrogenase , nucleotide , enzyme , chemistry , biology , gene , stereochemistry
For pyridine nucleotide‐dependent flavoezymes, binding both FAD and NAD(P)H on a single amino‐acids chain, we have found a high degree of internal sequence similarity for certain regions of FAD and NAD (P)H binding portions of the chain for any given protein. This was the case for a range of enzymes classes, including disulphide oxidoreductases (such as glutathione reductase, trypanothione reductase, lipoamide dehydrogenase, mercuric reductase), mono‐ and dioxygenases, nitrite reductaSE, alkyl hydroperoxidase and NADH dehydrogenase from E. coli . This provides strong support for gene duplication as the origin of at least part of the FAD and NAD(P)H recognising domains of such enzymes.