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Isolation and biochemical characterization of a zona pellucida‐binding glycoprotein of boar spermatozoa
Author(s) -
Jonáková Vèra,
Sanz Libia,
Calvete Juan J.,
Henschen Agnes,
Čechová Dana,
Töpfer-Petersen Edda
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80233-s
Subject(s) - zona pellucida , boar , glycoprotein , isolation (microbiology) , chemistry , zona pellucida glycoprotein , microbiology and biotechnology , biochemistry , oocyte , biology , semen , embryo , anatomy
Lectin‐like molecules on the sperm surface are implicated in the process of gamete recognition and adhesion. We have isolated and biochemically characterized a 15 kDa glycoprotein from ejaculated boar sperm which possess zona pellucida‐binding‐ and haemagglutinating‐activity. The zona/ 15 kDa protein interaction is inhibited by fucoldan, suggesting that the glycoprotein is one of the sperm components which participate in the initial gamete interaction. N‐Terminal sequence analysis of the isolated 15 kDa glycoprotein showed that it may belong to the same sperm/egg recognition‐mediating protein family as the sea urchin sperm protein binding.