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Interferon‐γ binds to heparan sulfate by a cluster of amino acids located in the C‐terminal part of the molecule
Author(s) -
Lortat-Jacob Hugues,
Grimaud Jean-Alexis
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80225-r
Subject(s) - heparan sulfate , chemistry , biochemistry , monoclonal antibody , amino acid , protease , cluster (spacecraft) , enzyme , stereochemistry , heparin , antibody , biology , genetics , computer science , programming language
Using three different approaches (domain mapping with monoclonal antibodies, limited enzymatic digestion and competition with synthetic peptides), we demonstrated that a cluster of basic amino acids on interferon‐γ is involved in its binding to heparan sulfate. This cluster (Lys 131 =Arg 121 ) is localized in the C‐terminal part of IFN‐γ. Once bound to heparan sulfate, IFN‐γ is protected against protease attack.