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High‐velocity mechanical DNA transfer of the chloramphenicolacetyl transferase gene into rodent liver, kidney and mammary gland cells in organ explants and in vivo
Author(s) -
Zelenin Alexander V.,
Alimov Andrey A.,
Titomirov Alexander V.,
Kazansky Alexander V.,
Gorodetsky Stanislav I.,
Kolesnikov Viktor A.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80212-l
Subject(s) - in vivo , microbiology and biotechnology , biology , kidney , explant culture , gene expression , gene , transfection , in vitro , endocrinology , biochemistry , genetics
Mouse and rat liver, kidney and mammary gland explants were bombarded with high‐velocity microprojectiles carrying a chloramphenicolacetyl transferase gene under different promoters (pTAT‐cat, p χ ‐Cusein‐cat, pβ‐Cusein‐cat). The expression of a CAT gene was revealed in all organ explants 24 h after transfection. The most pronounced expression was found when a TAT‐CAT construction was used. In experiments in vivo rat liver was bombarded in situ with microprojectiles carrying a pTAT‐cat DNA. A marked activity of the CAT gene was detected 24 h after the bombardment.