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The inhibition of restriction endonucleases due to Z‐DNA in negatively supercoiled plasmid
Author(s) -
Lesnik E.A.,
Bes'chetnikova Zh.A.,
Maslova R.N.,
Varschavsky Ja.M.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80211-k
Subject(s) - restriction enzyme , dna supercoil , plasmid , dna , endonuclease , cleavage (geology) , insert (composites) , microbiology and biotechnology , restriction map , restriction site , restriction fragment , chemistry , biology , genetics , dna replication , materials science , paleontology , fracture (geology) , composite material
Plasmid pGC20 containing the (dGC)??? insert in Sma 1 recognition site has been used to study the inhibition of cleavage by different restriction endonucleases due to Z‐DNA formation in (dCG) b10 sequence of the negatively supercoiled plasmid. Data obtained indicate the different sensitivity of restriction endonucleases to DNA conformational perturbations resulted from the Z‐DNA formation. Therefore, the inhibition of DNA cleavage by a particular restriction endonucleases cannot serve as a criterion for the estimation of the length of B—Z junctions in circular supercoiled DNAs