Premium
Demonstration of a transcription element in vitro between the capping site and translation initiation site of the mouse myelin basic protein gene
Author(s) -
Tamura Taka-aki,
Mikoshiba Katsuhiko
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80207-j
Subject(s) - transcription (linguistics) , myelin basic protein , gene , response element , microbiology and biotechnology , biology , in vitro , dna , promoter , chemistry , gene expression , myelin , genetics , central nervous system , philosophy , linguistics , neuroscience
A transcription element was identified, by in vitro analyses, just downstream from the capping site of the mouse myelin basic protein (MBP) gene. Deletion of this element caused a dramatic drop of transcription efficiency in mouse brain, rat liver and HeLa cell nuclear extracts, regardless of the form of DNA being closed circular or linear form. DNase I footprint analysis demonstrated the presence of a ubiquitous trans ‐acting factor for this region. This element functioned even when it is located in the normal direction downstream from the adenovirus major late promoter. Mutation analysis suggested that an essential part of the downstream element was located between +25 and +45.