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Effect of alanine insertion (P′ 5 ) on the reactive centre of α 1 ‐antitrypsin
Author(s) -
Avron Ann,
Reeve Francesca H.,
Lickorish Josephine M.,
Carrell Robin W.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80199-d
Subject(s) - recombinant dna , alanine , chemistry , serpin , mutant , microbiology and biotechnology , biochemistry , amino acid , biology , gene
A recombinant mutant of α 1 ‐antitrypsin with an inserted alanine in position P′ 1 (362=363) was compared with wild‐type recombinant and plasma α 1 ‐antitrypsins. Initial studies showed that contrary to other reports the wild recombinant inhibitor had the same, or even greater, association constants with trypsin and clastasex as the plasma inhibitor. The recombinant mutant as predicted had decreased inhibitory activity but no significant alteration in denaturation temperature and it retained the typical serpin S‐R change.