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Saturation of the processing of newly synthesized rRNA in isolated brain mitochondria
Author(s) -
Enriquez J.A.,
López-Pérez M.J.,
Montoya J.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80197-b
Subject(s) - incubation , rna , transcription (linguistics) , mitochondrion , ribosomal rna , biochemistry , biology , mitochondrial dna , microbiology and biotechnology , dna , messenger rna , in vivo , biophysics , chemistry , gene , genetics , philosophy , linguistics
Isolated rat brain mitochondria, when incubated in the presence of (α‐ 32 P)UTP in an appropriate incubation buffer, in which the energy requirements are provided by exogenous ADP in the presence of an oxidizable substrate, are able to support mitochondrial DNA transcription and RNA processing in a way faithfully resembling the in vivo process. Furthermore, we have strikingly found a saturation of the synthesis of mature 16 S and 12 S rRNA under conditions in which their RNA precursor as well as the mature mRNAs continue being synthesized. This suggests that synthesis of mature rRNAs could be regulated at the level of processing of their precursor rather than at the level of transcription.