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Light‐harvesting complexes of brown algae Biochemical characterization and immunological relationships
Author(s) -
Passaquet C.,
Thomas J.C.,
Caron L.,
Hauswirth N.,
Puel F.,
Berkuloff C.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80195-9
Subject(s) - fucoxanthin , violaxanthin , brown algae , algae , pigment , botany , chloroplast , biology , biochemistry , chemistry , carotenoid , lutein , zeaxanthin , gene , organic chemistry
The pigment composition of the light‐harvesting complexes isolated from several brown algae belonging to different orders has been analysed by reverse‐phase HPLC. Relative to whole chloroplasts, they were markedly enriched in Chl c , fucoxanthin and violaxanthin and conversely depleted in Chl a . The relative molar proportions of the 4 main pigments (Chl a /Chl e /fucoxanthin/violaxanthin) ranged from 100:18:76:6 to 100:30:107:17. The protein moiety of LH complexes of all the species studied were composed of one or two main polypeptide components in the range of 19–22 kDa. Those polypeptide subunits were arranged in polymeric particles about 240 kDa in Laminaria saccharina . A polyclonal antibody raised against the LH polypeptide of Fucus serratus has been tested on LH apoproteins of other Chromophytes and Chlorophytes. Phylogenic inplications of these results discussed.