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Saccharomyces cerevisiae gene SIT4 is involved in the control of glycogen metabolism
Author(s) -
Posas Francesc,
Clotet Josep,
Ariño Joaquin
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80183-4
Subject(s) - glycogen phosphorylase , glycogen synthase , phosphorylase kinase , glycogen debranching enzyme , biochemistry , gsk3b , glycogen branching enzyme , saccharomyces cerevisiae , mutant , glycogen , protein phosphatase 1 , phosphatase , chemistry , biology , microbiology and biotechnology , yeast , enzyme , gsk 3 , gene , phosphorylation
The gene SIT4 of S. cerevisiae , which codes for a protein structurally related to the catalytic subunit of mammalian protein phosphatase 2A, was disrupted in vitro. Analysis of glycogen synthase activity ratio in mutant haploid cells indicated that the enzyme was less active than in wild‐type cells. On the contrary, glycogen phosphorylase a activity was much higher. The activation of glycogen synthase observed in wild‐type cells after incubation with lithium ions was not detected in mutant cells. These results suggest that the product of gene SIT4, a putative protein phosphatase, could be involved in the control of glycogen metabolism in yeast cells.