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Saturation kinetics of palmitate uptake in perfused skeletal muscle
Author(s) -
Turcotte Lorraine P.,
Kiens Bente,
Richter Erik A.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80180-b
Subject(s) - kinetics , skeletal muscle , chemistry , biochemistry , saturation (graph theory) , enzyme kinetics , saturation vapor curve , membrane , biophysics , chromatography , biology , endocrinology , enzyme , physics , mathematics , quantum mechanics , combinatorics , active site
We investigated the kinetics of palmitate uptake in a physiological skeletal muscle preparation by using the isolated perfused rat hindquarter. When plotted against the unbound plasma palmitate concentration, palmitate uptake displayed a simple Michaelis‐Menten relation with a calculated V max and K m of 16.3 nmol · min −1 · g −1 and 0.06 μmol·l −1 , respectively. These results show that, as in isolated cell systems, uptake of free fatty acids in perfused skeletal muscle follows saturation kinetics consistent with carrier‐mediated membrane transport of free fatty acids.