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Phosphorylation and activation of hormone‐sensitive lipase in isolated macrophages
Author(s) -
Small Catherine A.,
Rogers M.Perenna,
Goodacre John A.,
Yeaman Stephen J.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80179-7
Subject(s) - hormone sensitive lipase , okadaic acid , incubation , lipase , phosphorylation , chemistry , hydrolase , enzyme , macrophage , hormone , cholesterol , biochemistry , phosphatase , medicine , endocrinology , biology , in vitro
Hormone‐sensitive lipase (HSL) is responsible for the neutral cholesterol ester hydrolase activity in macrophages. Incubation of intact WEHI macrophages or mouse peritoneal macrophages leads to phosphorylation of HSL, which is increased by incubation with either dibutyryl cyclic AMP and 3‐isobutyl‐1‐methylxanthine or okadaic acid. Correspondingly, these agents also activate neutral cholesterol ester hydrolase activity in intact WEHI cells. Regulation of mobilisation of esterified cholesterol in macrophages may be of antiatherogenic value, which this model system now allows us to investigate further.