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Inhibitory effect of calcium‐binding protein regucalcin on Ca 2+ ‐activated DNA fragmentation in rat liver nuclei
Author(s) -
Yamaguchi Masayoshi,
Sakurai Takashi
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80168-3
Subject(s) - dna fragmentation , calmodulin , microbiology and biotechnology , calcium , fragmentation (computing) , nad+ kinase , cytosol , biology , dna , calcium binding protein , biochemistry , chemistry , enzyme , apoptosis , programmed cell death , ecology , organic chemistry
Incubation of isolated rat liver nuclei with ATP, NAD + , and micromolar Ca 2+ concentrations of various metal ions resulted in extensive DNA hydrolysis. Half‐maximal activity occurred with 1.0 μM Ca 2+ added, and saturation of the process was observed with 10 μM Ca 2+ , The Ca 2+ (10 μM)‐activated DNA fragmentation was inhibited by the presence of Ca 2+ ‐binding protein regucalcin isolated from rat liver cytosol. The inhibitory effect of regucalcin was complete at 0.5 μM. At 25μM Ca 2+ added, such an effect of regucalcin (1.0μM) was not seen, Regucalcin also inhibited Ca 2+ ‐activated DNA fragmentation in the presence or calmodulin (10 and 20 μg). The results show that regucalcin can inhibit the Ca 2+ ‐activated DNA fragmentation due to binding the metal, suggesting a role in regulation of liver nuclear functions.