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B—Z transition of poly(dG‐m 5 dC) induced by binding of Lys‐containing peptides
Author(s) -
Takeuchi Hideo,
Hanamura Nobuyuki,
Hayasaka Hitoshi,
Harada Issei
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80161-u
Subject(s) - chemistry , transition (genetics) , binding energy , crystallography , stereochemistry , physics , biochemistry , atomic physics , gene
Effects of oligopeptides containing Lys residues on the conformation of poly(dG‐m 5 dC) have been investigated by circular dichroism spectroscopy. Lys‐Ala‐Lys (KAK) and its longer analogs with Lys‐Ala repeats are found to convert the B‐form polynucleotide to the Z form very efficiently. The ability to induce the B‐Z transition is characteristic of alternating Lys‐Ala sequences and increases exponentially with increasing number of the repeats. The heptupeptide KAKAKAK has an ability comparable with that of spermine, one of the most effective inducers hitherto known. The present results provide the list example of the B‐Z transition of poly(dG‐m 5 dC) induced by peptide binding.