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Activation of phospholipase D in rabbit neutrophils by fMet‐Leu‐Phe is mediated by a pertussis toxin‐sensitive GTP‐binding protein that may be distinct from a phospholipase C‐regulating protein
Author(s) -
Kanaho Yasunori,
Kanoh Hiroyuki,
Nozawa Yoshinori
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80160-5
Subject(s) - pertussis toxin , g protein , phospholipase c , gtp' , gtpase , phospholipase , chemistry , gtp binding protein regulators , toxin , phospholipase d , biochemistry , receptor , enzyme
Stimulation by N ‐formyl‐Met‐Leu‐Phe (fMLP) of rabbit peritoneal neutrophils, in which phosphatidylcholine was preferentially labeled with l‐ O ‐[ 3 H]octadecyl lyso platelet‐activating factor, activated phospholipase D, resulting in the formation of [ 3 H]PA from [ 3 H]PC. A direct activator of GTP‐binding proteins (G‐proteins), NaF, also stimulated [ 3 H]PA formation. fMLP‐stimulated [ 3 H]PA formation was inhibited by pertussis toxin (IAP) in a time‐ and dose‐dependent manner. IAP also inhibited fMLP‐stimulated IP 3 formation, but the inhibition of IP 3 formation was significantly greater than that of [ 3 H]PA formation. These results indicate that activation of phospholipase D by fMLP in rabbit neutrophils is mediated by an IAP‐sensitive G‐protein that may be distinct from a phospholipase C‐regulating protein.