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Oxidation/reduction explains heterogeneity of pancreatic somatostatin
Author(s) -
Bersani Maurizio,
Johnsen Anders H.,
Holst Jens Juul
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80157-x
Subject(s) - somatostatin , size exclusion chromatography , chemistry , homogeneous , acetic acid , elution , chromatography , biochemistry , medicine , enzyme , physics , thermodynamics
Somatostatin 14 (SS 14) has been isolated from pancreatic extracts, but open gel filtration immunoreactive SS often elutes in two peaks. We isolated both peaks, but upon sequence analysis only authentic SS 14 could be identified. By further gel filtration experiments it turned out that both synthetic and extractable SS appeared homogeneous at neutral pH 7.5, but showed an additional earlier peak in acetic acid. After addition of mercaptoethanol, all of the SS eluted at this earlier position regardless of the pH. We conclude that partial reduction/oxidation of SS explains the heterogeneity.