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Identification of a receptor protein for neuropeptide Y in rabbit kidney G‐protein association and inhibition of adenylate cyclase
Author(s) -
Gimpl Gerald,
Wahl Jochen,
Lang Rudolf E.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80153-t
Subject(s) - adenylate kinase , cyclase , receptor , neuropeptide y receptor , g protein , g protein coupled receptor , endocrinology , chemistry , neuropeptide , kidney , medicine , microbiology and biotechnology , biology , biochemistry
We previously identified the receptor for neuropeptide Y (NPY) on rabbit kidney membranes as a 100 kDa protein [(1990) J. Biol. Chem. 265, 18142–18143]. It is demonstrated in this study that the stable guanine nucleotide analogue, guanosine 5′‐ O ‐(3‐thiotriphosphate)(GTP γ S), is capable of decreasing the labeling of the 100 kDa protein in a concentration‐dependent manner, with an IC 20 value of 50 μM. This suggests that the 100 kDa protein represents a G‐protein coupled receptor type. In isolated proximal tubulex from rabbit kidney NPY decreases the parathyroid hormone stimulated cAMP production in a dose‐dependent fashion. This indicates that the NPY receptor in rabbit kidney is negatively coupled to adenylate cyclase by a G 1 ‐like protein.