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The nirSTBM region coding for cytochrome cd 1 ‐dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono‐, di‐, and tetraheme proteins
Author(s) -
Jüngst Angelika,
Wakabayashi Sadao,
Matsubara Hiroshi,
Zumft Walter G.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80150-2
Subject(s) - pseudomonas stutzeri , chemistry , cytochrome , cytochrome c , respiration , nitrite , biochemistry , biology , nitrate , apoptosis , botany , organic chemistry , bacteria , genetics , enzyme
Genes for respiratory nitrite reduction (denitrification) of Pseudomonas stutzeri are clustered within 7 kbp. A 4.6‐kbp Hind III‐ Kpn I fragment carrying nirS , the structural gene for cytochrome cd 1 , was sequenced. An open reading frame immediately downstream of nirS codes for a 22.8‐kDa protein with four heme c ‐binding motifs. Mutagenesis of this gene causes an apparent defect in electron donation to cytochrome cd 1 . Following this ORF are the structural genes for cytochrome c 552 , cytochrome c 551 , and ORF5 that codes for a 11.9‐kDa monoheme protein. All cytochromes have a signal sequence for protein export.