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In vivo promoter activity of the synthetic Pribnow box
Author(s) -
Koroleva O.N.,
Drutsa V.L.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80118-m
Subject(s) - in vivo , chemistry , synthetic biology , computational biology , biology , genetics
The synthetic polydeoxyribonucleotides TGCA(TTATAATGCA), ??? a containing the consensus Pribnow box, were inserted upstream of the promoterless gal operon in a specially constructed plasmid. Using the MacConkey agar indicator technique it has been show that these inserts are able to induced an efficient in vivo expression of the gal operon.