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Biochemical evidence for histidine oxidation in photosystem II depleted of the Mn‐cluster for O 2 ‐evolution
Author(s) -
Ono Taka-aki,
Inoue Yorinao
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80112-g
Subject(s) - photosystem ii , histidine , cluster (spacecraft) , chemistry , photosynthesis , photosystem i , biophysics , biochemistry , biology , enzyme , computer science , programming language
Flash excitation of Tris‐treated photosystem II (PSII) membranes generated a thermoluminescene band peaking ‐25°C. Indicating that a positively charged oxidizing equavalent is stably accumulated on donor side of PSII even in the Mn‐cluster. This oxidizing equivalent was sensitive to low concentrations of exogenous Mn ??? , and its stable accumulation was reversibly inhibited by diethylpyrocarbonate treatment that modifies histidine residues with high specificity. It was inferred that there is a photooxidizable histidine residue(s) on donor side of PSII and it provides a redox‐active ligand for Mn