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Identification of sulfurtransferase enzymes in Azotobacter vinelandii
Author(s) -
Pagani S.,
Franchi E.,
Colnaghi R.,
Kennedy C.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80105-c
Subject(s) - sulfurtransferase , rhodanese , azotobacter vinelandii , fast protein liquid chromatography , chemistry , biochemistry , enzyme , ion chromatography , chromatography , organic chemistry , nitrogenase , cysteine , nitrogen fixation , nitrogen
Rhodanese and 3‐mercaptopyruvate sulphurtransferase have been identified in A. vinelandii . Two distinct active fractions of the two sulphurtransferases were obtained after FPLC ion‐exchange chromatography of material partially purified from crude extracts. Rhodanese has been purified to homogeneity, and it consists of one polypeptide chain of M r ca 25 000. A partial purification of 3‐mercaptopyruvate sulphurtransferase was obtained.