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Effect of α‐ketoglutarate and its structural analogues on hysteretic properties of α‐ketoglutarate dehydrogenase
Author(s) -
Bunik V.I.,
Romash O.G.,
Gomazkova V.S.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80104-b
Subject(s) - chemistry , dehydrogenase , catalysis , enzyme , product inhibition , stereochemistry , biochemistry , biophysics , biology , non competitive inhibition
The burst of product accumulation during the KGD reaction was investigated. It has been shown not to be the obligatory feature of catalysis, but appears when increasing the enzyme saturation by KG. Structural analogues of KG and the SH‐group modification suppress the initial burst without preventing catalysis. The results obtained are in favour of the existence of the regulatory site for binding KG and its structural analogues essential for hysteretic properties of KGD.