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Cooperative B—Z transition of poly(dG—dC) induced by spermine at physiological temperature in aqueous medium
Author(s) -
M. V. R. Rao,
M. Atreyi,
Shashi Saxena
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80084-g
Subject(s) - spermine , aqueous solution , chemistry , amine gas treating , glass transition , ionic strength , enthalpy , transition (genetics) , biophysics , polymer chemistry , biochemistry , organic chemistry , thermodynamics , polymer , biology , physics , gene , enzyme
Spermine induced B—Z transition of poly(dG—dC) in aqueous medium at and above physiological temperature at cellular concentration levels in low ionic strength medium. The amine to phosphate ratio, A P, at the midpoint of the transition decrease with increase in temperature. The enthalphy change was 45 ± 8 Kcal per mol of cooperative unit; the transition induced by spermine is highly cooperative with a cooperative unit length of 700 ± 20 at 50°C.