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The rat α 2 ‐C4 adrenergic receptor gene encodes a novel pharmacological subtype
Author(s) -
Mark M. Voigt,
Susan K. McCune,
R Y Kanterman,
Christian C. Felder
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80080-m
Subject(s) - adrenergic receptor , alpha 1d adrenergic receptor , gene , alpha 1b adrenergic receptor , yohimbine , microbiology and biotechnology , gene product , receptor , biology , complementary dna , endocrinology , alpha (finance) , beta 3 adrenergic receptor , alpha 1a adrenergic receptor , gene expression , beta 1 adrenergic receptor , medicine , biochemistry , antagonist , construct validity , nursing , patient satisfaction
A rat gene and brain cDNA (pA2d) encoding the homologue of the human α‐C4 adrenergic receptor subtype were isolated and characterized. RNA blots indicate that this gene is expressed in brain, heart and kidney but not in lung, liver or pancreas. Yohimbine, WB‐4101 and prasozin all exhibited high affinity for this receptor in binding studies. Clonidine was more potent and efficacious than norepinephrine in inhibiting forskolin‐stimulated cAMP production in CHO cells expressing pA2d. Together, these data suggest that the α 2 ‐C4 gene product defines a previously undescribed pharmacological subtype α 2 ‐adrenergic receptor.