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Studies on the distribution of cellular myosin with antibodies to isoform‐specific synthetic peptides
Author(s) -
Noriko Murakami,
Padmaja Mehta,
Marshall Elzinga
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80074-d
Subject(s) - gene isoform , polyclonal antibodies , myosin , peptide , antibody , antiserum , microbiology and biotechnology , biochemistry , biology , chemistry , immunology , gene
Antisera were produced against two synthetic peptides having sequences specific for cellular myosin heavy chains from human macrophages (peptide IIA) and bovine brain (peptide IIB). Immunoblots of tissue extracts were made with these antibodies, and they showed that mammalian cells have at least three distinct cellular myosin heavy chain isoforms. Two of the isoforms (MIIB 1 and MIIB 2 ) were recognized by antibodies against the peptide IIB, and the other (MIIA) by the anti‐peptide IIA antibodies. Polyclonal anti‐platelet myosin antibodies recognized the MIIA isoform, but did not recognize MIIB 1 or MIIB 2 . The isoforms were expressed in a tissue‐specific pattern; the MIIB 1 isoform was found only in brain.