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Solubilization of rat kidney lysosomes in reversed micelles of aerosol OT in octane
Author(s) -
Pshezhetsky Alexey V.,
Buneeva Olga A.,
Wiederschain German Ya.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80055-8
Subject(s) - solubilization , micelle , chemistry , octane , kidney , aerosol , chromatography , biochemistry , biology , endocrinology , organic chemistry , aqueous solution
Intact lysosomes from rat kidneys were solubilized in the ternary system: surfactant (Aerosol OT)‐buffer‐organic solvent. According to data of laser light‐scattering analysis and kinetic experiments with the lysosomal marker enzyme, N ‐acetyl‐β‐D‐hexosaminidase (EC 3.2.1.30), the solubilization of lysosomes in this system resulted in the destruction of the lysosomes and the entrapping of their components in reversed micelles.