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Site‐directed mutagenesis of La protease
Author(s) -
Amerik Alexander Yu.,
Antonov Vladimir K.,
Gorbalenya Alexander E.,
Kotova Svetlana A.,
Rotanova Tatyana V.,
Shimbarevich Elena V.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80053-6
Subject(s) - site directed mutagenesis , mutagenesis , mutant , protease , escherichia coli , biochemistry , chemistry , enzyme , microbiology and biotechnology , biology , gene
Comparative sequence analysis of Escherichia coli ATP‐dependent La protease led to the suggestion that Ser 679 is the catalytically active enzyme residue. Site‐directed mutagenesis Ser 679 → Ala, investigation of the cells containing the mutant plasmid, and study of the partially purified mutant protein produced results in favour of this suggestion.