Premium
Induction of differentiation of human leukemia cells by inhibitors of myosin light chain kinase
Author(s) -
Makishima Makoto,
Honma Yoshio,
Hozumi Motoo,
Sampi Kazumi,
Hattori Masao,
Motoyoshi Kazuo
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80044-4
Subject(s) - myosin light chain kinase , chemistry , k562 cells , kinase , microbiology and biotechnology , calmodulin , immunoglobulin light chain , protein kinase a , activator (genetics) , protein kinase c , sulfonyl , leukemia , myosin , biochemistry , biology , enzyme , in vitro , antibody , receptor , immunology , alkyl , organic chemistry
Inhibitors of myosin light chain kinase, 1‐(5‐chloronaphthalene‐1‐sulfonyl)‐1 H ‐hexanydro‐1,4‐diazepine hydrochloride (ML‐9) and 1‐(5‐iodonaph‐thalene‐1‐sulfonyl)‐1 H ‐hexahydro‐1,4‐diazepine hydrochloride (ML‐7), induced Nitroblue tetrazolium reducing activity, lysozyme activity and morphological maturation of human monoblastic U937, THP‐1 and promyelocytic HL‐60 cells, but not of erythroblastic K562 cells. However, three analogs of ML‐9, which are an inhibitor and an activator of protein kinase C, and a calmodulin antagonist, respectively, did not induce differentiation of the cells.