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Widespread distribution of Gqα/Gllα detected immunologically by an antipeptide antiserum directed against the predicted C‐terminal decapeptide
Author(s) -
Mitchell Fiona M.,
Mullaney lan,
Godfrey Philip P.,
Arkinstall Steve J.,
Wakelam Michael J.O.,
Milligan Graeme
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80043-3
Subject(s) - antiserum , chemistry , terminal (telecommunication) , biology , microbiology and biotechnology , immunology , antigen , telecommunications , computer science
Antisera were raised to a synthetic peptide which represents the predicted C‐terminal decapeptide of the α subunit of the G‐proteins Gq and Gll. Competitive ELISA indicated that antiserum CQ2 displayed strong reactivity against this peptide. Antiserum CQ2 identified an apparently single polypeptide of 42 kDa which was expressed widely. The mobility of this polypeptide in SDS‐PAGE was not modified by pretreatment of cells with pertussis toxin, indicating that it was not a substrate for this toxin. Furthermore, the levels and mobility of this polypeptide were unaltered by treatment of cells with cholera toxin, defining that it was not related to G s α.