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Mutant analysis of interaction of the Bacillus subtilis transcription regulator AbrB with the antibiotic biosynthesis gene tycA
Author(s) -
Fürbaβ Rainer,
Marahiel Mohamed A.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80038-5
Subject(s) - bacillus subtilis , gene , mutant , promoter , transcription (linguistics) , biology , genetics , binding site , regulator , lac operon , response regulator , transcription factor , microbiology and biotechnology , escherichia coli , gene expression , bacteria , linguistics , philosophy
The AbrB protein of B. subtilis represses the transcription of various postexponentially expressed genes, such as the antibiotic biosynthesis gene tycA . Recently, we have shown that AbrB binds to the tycA promoter region at two A+T‐rich sites; the ‘promoter site’ (−60 to −35) and the ‘leader site’ (+169 to +231). In this study we demonstrate that a P tyc — lacZ fusion missing the leader region is constitutively expressed in wild‐type B. subtilis cells and in B. subtilis cells carrying spoOA or abrB mutations. We also show that substitution mutations within the recently reported potential helix‐turn‐helix DNA binding motif of AbrB did not affect its specific DNA binding ability.