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Phytohormonal regulation of S‐adenosylmethionine synthetase and S‐adenosylmethionine levels in dwarf pea epicotyls
Author(s) -
Mathur Mukul,
Sachar R.C.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80028-2
Subject(s) - chemistry , biology
A significant stimulation (2‐ to 2.5‐fold) of AdoMet synthetase was witnessed in glibberellicd acid (GA 3 , 1μM)‐treated epicotyls of the dwarf pea ( Pisum sativum ). This was accompanied by a 2.4‐fold increase in the endogenous pool of S‐adenosylmethionine. Both abscisic acid (10 μM) and cycloheximide (20 ) inhibited the GA3‐mediated enhancement of AdoMet synthetase activity. Three isozymes of AdoMet synthetase were detected in GA 3 ‐treated epicotyls, whereas a single activity peak was observed in controls. Thus, GA 3 seems to control the induction of two new isozymes of AdoMet synthetase in the dwarf pea. By contrast, the tall pea exhibited three isozymes of AdoMet synthetase even in the absence of GA 3 treatment. High concentration of L‐methionine (2 mM) mimicked the GA 3 ‐elicited induction of two new isozymes of AdoMet synthetase in dwarf pea epicotyls.