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Magnetic resonance studies of murine macrophages
Author(s) -
King Nicholas J.C.,
Ward Mary H.,
Holmes Kerry T.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80024-w
Subject(s) - nuclear magnetic resonance , chemistry , magnetic resonance imaging , biophysics , biology , medicine , physics , radiology
Proton magnetic resonance spectroscopy ( 1 H‐MRS) was used to investigate the membranes of macrophages activated by γ‐interferon in vitro and by Listeria monocytogenes in vivo. We report the appearance with activation, of a high resolution spectrum indistinguishable from that found in activated T and B cells and embryonic and malignant cell types previously studied. We furthermore show that proliferation is not a prerequisite for the appearance of this activated spectrum. This supports the idea that membrane ‘activation’ in all cells, irrespective of origin, may be accompanied by similar architectural changes, and suggests that a common pathway exists for the activation of cell membranes of the immune system, possibly important in the acquisition of increased motility. The use of 1 H‐MRS as a non‐invasive tool for analysis of activation is discussed.