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Dinucleoside tetraphosphatase from human blood cells
Author(s) -
Pinto Rosa Maria,
Costas Maria Jesús,
Fernández Ascensión,
Canales José,
García-Agundez José Augusto,
Cameselle José Carlos
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80021-t
Subject(s) - human blood , chemistry , ammonium sulfate , biochemistry , adenosine , cell , homogeneous , ligand (biochemistry) , elution , chromatography , microbiology and biotechnology , biology , receptor , physiology , physics , thermodynamics
Dinucleoside tetraphosphatase (Np4Nase; EC 3.6.1.17) has been purified 170000‐fold from a 30–60% ammonium sulfate fraction of a human blood cell extract. Purification included a dye‐ligand affinity elution step using the inhibitor adenosine 5'‐tetraphosphate. Human blood Np4Nase resembled rat liver Np4Nase, including recognition by anti‐rat Np4Nase, but differed from homogeneous human leukemia Np4Nase in the 1000‐fold lower specific activity of the latter. The results are discussed in relation to the potential role of diadenosine tetraphosphate (Ap4A) in the control of cell division and the turnover of Ap4A in blood.