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Comparative analysis of mitochondrial and amyloplast adenylate translocators
Author(s) -
Pozueta-Romero Javier,
Viale A.M.,
Akazawa T.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80016-v
Subject(s) - amyloplast , adenylate kinase , chemistry , mitochondrion , microbiology and biotechnology , biochemistry , biology , chloroplast , plastid , enzyme , gene
Structurally intact and metabolically competent mitochondria isolated from liquid‐culture cells of sycamore ( Acer pseudoplatanus L.) were shown to incorporate ADPglucose. Employing the double silicone oil layer filtering centrifugation method, we examined the kinetic properties of the uptake of various adenylates as well as the inhibitory effects exerted by carboxyatractyloside, atractyloside and bongkrekic acid, known specific inhibitors of the mitochondrial adenylate translocator. Immunoblot patterns of peptides derived from the partial proteolytic digestion of the mitochondrial and plastid adenylate translocators were shown to be essentially the same. We conclude that the molecular entities engaged in the adenylate transport system operating in two different organelles. mitochondria and amyloplasts, are very similar.