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Molecular cloning of the Ecotin gene in Escherichia coli
Author(s) -
Lee Hae Ryun,
Seol Jae Hong,
Kim Ok Mae,
Lee Cheol Soon,
Suh Se Won,
Hong Yeong-Man,
Tanaka Keiji,
Ichihara Akira,
Ha Doo Bong,
Chung Cin Ha
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80014-t
Subject(s) - biology , gene , molecular cloning , escherichia coli , genetics , nucleic acid sequence , microbiology and biotechnology , coding region , peptide sequence
The nucleotide sequence of a 876 bp region in E. coli chromosome that encodes Ecotin was determined. The proposed coding sequence for Ecotin is 486 nucleotides long, which would encode a protein consisting of 162 amino acids with a calculated molecular weight of 18 192 Da. The deduced primary sequence of Ecotin includes a 20‐residue signal sequence, cleavage of which would give rise to a mature protein with a molecular weight of 16 099 Da. Ecotin does not contain any consensus reactive site sequences of known serine protease inhibitor families, suggesting that Ecotin is a novel inhibitor.