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Cloning and expression of human endothelin‐1 receptor cDNA
Author(s) -
Hosoda Kiminori,
Nakao Kazuwa,
Suga Shin-ichi,
Ogawa Yoshihiro,
Mukoyama Masashi,
Shirakami Gotaro,
Saito Yoshihiko,
Nakanishi Shigetada,
Imura Hiroo
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80007-p
Subject(s) - complementary dna , microbiology and biotechnology , biology , xenopus , northern blot , cdna library , receptor , endothelin receptor , gene , biochemistry
We isolated a human endothelin‐1 (ET‐1) receptor cDNA from a human placenta cDNA library. The cDNA encodes a 427‐amino acid protein with seven putative transmembrane domains. The rank order of the binding to the receptor expressed in COS‐7 cells was: ET‐1 ⩾ ET‐2 ⪢ ET‐3. The receptor expressed in Xenopus oocytes showed a potent electrophysiological response to 1 × 10 −7 M ET‐1 under voltage clamp at −60 mV, while a much weaker response was produced by 1 × 10 −7 M ET‐3. Northern blot analysis with RNA from human tissues revealed a single band with a size of 4.3 kb in a wide variety of human tissues, especially highly in the blood vessel.