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Identical genes for trimethoprim‐resistant dihydrofolate reductase from Staphylococcus aureus in Australia and Central Europe
Author(s) -
Burdeska Alexander,
Ott Michael,
Bannwarth Willi,
Then Rudolf L.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81529-w
Subject(s) - dihydrofolate reductase , staphylococcus aureus , plasmid , ecori , biology , bglii , nucleic acid sequence , gene , microbiology and biotechnology , trimethoprim , transposable element , genetics , genome , antibiotics , bacteria
The nucleotide sequence of a 1.25 kb Bgl II/ Eco RI fragment from the 34 kb trimethoprim (Tp)‐resistant plasmid pABU1 of Staphylococcus aureus 157/4696, isolated in Zürich, was determined. It contained the entire Tp‐resistant dihydrofolate reductase gene, 197 bp of the thymidylate synthetase, 395 bp of a truncated gene and 111 bp of IS257R1. With the exception of one single base pair at position 862 the sequence of the whole fragment was identical to nucleotides 1633 to 2885 of the Tp‐resistant transposon Tn 4OO3 in plasmid pSK 1 from an Australian S. aureus isolate. This suggests the worldwide dissemination of Tn 4OO3 in multiresistant Staphylococci .