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Footprinting studies on the sequence‐selective binding of pentamidine to DNA
Author(s) -
Fox Keith R.,
Sansom Clare E.,
Stevens Malcolm F.G.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81527-u
Subject(s) - pentamidine , micrococcal nuclease , footprinting , chemistry , dna , nuclease , cleavage (geology) , binding site , base pair , dna footprinting , deoxyribonuclease i , stereochemistry , biochemistry , microbiology and biotechnology , base sequence , biology , dna binding protein , nucleosome , gene , pneumonia , histone , medicine , transcription factor , paleontology , fracture (geology)
The sequence‐selective binding of pentamidine, an antimicrobial aromatic diamidine, has been investigated by footprinting studies on two different DNA fragments using DNase I, micrococcal nuclease and hydroxyl radical as probes. Each probe reveals drug‐induced protection from cleavage in AT‐rich regions. The best binding sites consist of at least 5 consecutive AT base pairs. Three or less AT pairs do not constitute a pentamidine binding site.