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NMR studies of the lipid metabolism of T47D human breast cancer spheroids
Author(s) -
Ronen S.M.,
Stier A.,
Degani H.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81526-t
Subject(s) - spheroid , phosphatidylethanolamine , phosphatidylcholine , phospholipid , phosphorylcholine , chemistry , in vivo , membrane , metabolism , phosphocholine , lipid metabolism , human breast , in vitro , cancer cell , biochemistry , biology , cancer , genetics , microbiology and biotechnology
The in vivo 31 P NMR spectrum of T47D human breast cancer cells grown as spheroids shows changes in the phosphomonoester lipid precursors as a function of spheroid size. The ratio of phosphorylethanolamine (PE) to phosphorylcholine (PC) was 1.0±0.3 for 3‐day‐old, 150 μm spheroids. This ratio increased to 2.4±0.4 for spheroids 7 days and older and which were at least 300 μm in diameter. To investigate the phosphatidylethanolamine to phosphatidylcholine (PdyIE/Pdy1C) ratio in the membranes, chloroform/methanol extracts of spheroids were performed. The 31 P spectrum of these extracts showed no change with spheroid size, namely the PdyIE/Pdy1C ratio was 0.5±0.06 for spheroids of all ages suggesting that membrane composition is strongly regulated at the precursor level.