Premium
Subcellular distribution of α, and β subunit proteins of Ca 2+ /calmodulin‐dependent protein kinase II expressed in Chinese hamster ovary cells
Author(s) -
Yamauchi Takashi,
Sekihara Shun-ichi,
Ohsako Shunji
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81505-i
Subject(s) - chinese hamster ovary cell , protein subunit , subcellular localization , calmodulin , biology , g alpha subunit , protein kinase a , biochemistry , microbiology and biotechnology , interleukin 10 receptor, alpha subunit , phosphorylation , enzyme , cytoplasm , gene , receptor
When two cDNAs respectively encoding the entire coding regions of α and β subunits of Ca 2+ /calmodulin‐dependent protein kinase II (CaM kinase II) were introduced into Chinese hamster ovary cells, the expressed α and β subunits were differently associated with subcellular structure. Although α subunit was loosely associated with subcellular structure, about 80% of CaM kinase II acitivity of α subunit was found in soluble fraction. More than 50% of the β subunit bound to the membrane, and the remainder was soluble but was loosely associated with subcellular structure. The relative rate of phosphorylation for substrate proteins of the β subunit bound to membrane was significantly different from that of the soluble form.