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Reduced levels of ADP‐ribosylatable elongation factor‐2 in aged and SV40‐transformed human cell cultures
Author(s) -
Riis Bent,
Rattan Suresh I.S.,
Derventzi Anastassia,
Clark Brian F.C.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81502-f
Subject(s) - elongation factor , elongation , ageing , diphtheria toxin , eukaryotic translation elongation factor 1 alpha 1 , microbiology and biotechnology , cell culture , in vitro , wi 38 , cell , transformation (genetics) , cell cycle , chemistry , biology , biochemistry , biophysics , toxin , ploidy , genetics , materials science , ribosome , rna , ultimate tensile strength , metallurgy , gene
The elongation step is involved in the regulation of protein synthesis during the cell cycle, environmental stress, ageing and transformation. Using a diphtheria toxin‐mediated assay for measuring the levels of ADP‐ribosylatable elongation factor EF‐2, we have observed an irreversible decrease of up to 64% in the amount of ADP‐ribosylatable EF‐2 in normal diploid human fibroblasts MRC‐5 undergoing ageing in vitro. However, a similar decrease in low serum‐associated G 0 /G 1 ‐arrested cells is reversible both in MRC‐5 cells and in their SV40‐transformed counterparts. Reduced levels of ADP‐ribosylatable EF‐2 could account for the slowing‐down of protein synthesis during cell cycle arrest and during cellular ageing in culture.