Premium
Separation of two βγ subunit complexes of brain GTP‐binding proteins composed of distinct γ subunits
Author(s) -
Asano Tomiko,
Morishita Rika,
Kobayashi Tamio,
Kato Kanefusa
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81501-e
Subject(s) - protein subunit , gtp' , interleukin 10 receptor, alpha subunit , gamma aminobutyric acid receptor subunit alpha 1 , gi alpha subunit , biochemistry , specificity factor , chemistry , biology , g alpha subunit , enzyme , gene , polymerase , rna dependent rna polymerase
The γ subunits of GTP‐binding proteins are always associated with β subunits under physiological conditions, and at least two γ subunits exist in the brain. We report here that brain βγ subunit complexes composed of distinct γ subunits can be separated by anion exchange chromatography under nondenaturing conditions. One βγ complex was composed of a 36‐kDa β subunit and a 6‐kDa γ subunit and the other was composed of 36/35‐kDa β subunits and 4.5‐kDa γ subunit. The 6‐kDa γ subunit was phosphorylated by protein kinase C but the 4.5‐kDa γ subunit was not.