Premium
Formylmethanofuran: Tetrahydromethanopterin formyltransferase from Methanosarcina barkeri Identification of N 5 ‐formyltetrahydromethanopterin as the product
Author(s) -
Breitung J.,
Thauer R.K.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81477-6
Subject(s) - methanosarcina barkeri , chemistry , identification (biology) , biochemistry , biology , botany , organic chemistry , methane , methanogenesis
Formylmethanofuran: tetrahydromethanopterin formyltransferase was purified from methanol grown Methanosarcina barkeri to apparent homogeneity and characterized with respect to its molecular and kinetic properties. The enzyme was found to be very similar to the formyltransferase from H 2 /CO 2 grown Methanobacterium thermoautotrophicum . It also catalyzed the formation of N 5 ‐formyltetrahydromethanopterin rather than of N 10 ‐formyltetrahydromethanopterin from formylmethanofuran and tetrahydromethanopterin.