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Mutagenesis of the gene encoding amicyanin of Paracoccus denitrificans and the resultant effect on methylamine oxidation
Author(s) -
van Spanning Rob J.M.,
Wansell Corry W.,
Reijnders Willem N.M.,
Oltmann L.Fred,
Stouthamer Adrian H.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81475-4
Subject(s) - paracoccus denitrificans , gene , biochemistry , protein subunit , biology , periplasmic space , copper protein , microbiology and biotechnology , chemistry , enzyme , escherichia coli , copper , organic chemistry
The gene encoding the blue‐copper protein amicyanin was isolated from a genomic bank of Pracoccus denitrificans by using a synthetic oligonucleotide. It is located directly downstream of the gene encoding the small subunit of methylmine dehydrogenase. Amicyanin is transcribed as a presursor protein with a signal sequence, typical for periplasmic proteins. Specific inactivation of amicyanin by means of gene replacement techniques resulted in the complete loss of the ability to grow on methylamine.