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InsP 3 ‐ and Ca 2+ ‐induced Ca 2+ release in single mouse oocytes
Author(s) -
Peres Antonio
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81474-3
Subject(s) - inositol , chemistry , intracellular , biophysics , kinetics , calcium , isotopes of calcium , biochemistry , biology , receptor , physics , organic chemistry , quantum mechanics
To better understand the mechanism of intracellular Ca 2+ mobilization, mouse oocytes were micro‐injected with ‘caged’‐inositol‐1,4,5 trisphosphate (caged‐InsP 3 ) together with the Ca 2+ indicator Fluo‐3 ??? and monitor Ca 2+ redistribution. Photo‐released InsP 3 olicits [Ca 2+ ], changes exhibiting several kinetics phases and threshold ??? oscillations were induced after a single InsP 3 pulse. Autoregenerative Ca 2+ transients could also be induced by injections of Ca 2+ itself, demonstrating unequivocally the presence of a Ca 2+ ‐induced Ca 2+ ‐release mechanism in these cells.