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Chemoattractant‐induced membrane hyperpolarization in Dictyostelium discoideum A possible role for cyclic GMP
Author(s) -
Van Duijn Bert,
Wang Mei
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81471-y
Subject(s) - hyperpolarization (physics) , dictyostelium discoideum , chemotaxis , stimulation , membrane potential , biophysics , microbiology and biotechnology , potassium channel , chemistry , slime mold , biology , biochemistry , neuroscience , receptor , stereochemistry , nuclear magnetic resonance spectroscopy , gene
Membrane potentials of the cellular slime mold Dictyostelium discoideum were monitored after chemotactic stimulation by measuring the distribution of the lipophilic cation tetraphenylphosphonium. Stimulation with the chemoattractant cAMP induces a transient membrane hyperpolarization which reaches its most negative value between 1 3 min after stimulation. This hyperpolarization is consistent with the opening of potassium channels. Measurements in streamer F mutant cells reveal that cGMP likely plays a role in the regulation of the cAMP‐induced hyperpolarization.