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Synchronized repetitive spikes in cytoplasmic calcium in confluent monolayers of human umbilical vein endothelial cells
Author(s) -
Neylon Craig B.,
Irvine Robin F.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81465-z
Subject(s) - umbilical vein , endoplasmic reticulum , calcium , biophysics , cytoplasm , histamine , caffeine , microbiology and biotechnology , chemistry , prostacyclin , endothelium , inositol , endothelins , biology , biochemistry , endothelin receptor , endocrinology , in vitro , receptor , organic chemistry
Synchronized repetitive spikes in cytoplasmic free calcium concentration, [Ca 2+ ] i , are evoked by histamine in confluent monolayers of human endothelial cells. The repetitive spikes, which are apparently dependent upon the establishment of cell coupling, are also induced by caffeine, indicating that they may be due to an oscillatory release of Ca 2+ from the endoplasmic reticulum, and may not involve oscillations in inositol phosphates. It is suggested that sunchronized repetitive spikes in [Ca 2+ ] i might lead to oscillatory release of endothelial‐derived substances such as prostacyclin, nitric oxide and endothelin, which have potent effects on the vascular system.