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Morphological alteration of Xenopus oocytes induced by valine‐14 p21 rho depend on isoprenylation and are inhibited by Clostridium botulinum C3 ADP‐ribosyltranferase
Author(s) -
Mohr Chistiane,
Just Ingo,
Hall Alan,
Aktories Klaus
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81464-y
Subject(s) - microinjection , xenopus , prenylation , gtp' , chemistry , microbiology and biotechnology , biology , biochemistry , enzyme , gene
Microinjection of the constitutively active recombinant Val‐14 p21rho A into Xenopus oocytes induced dramatic morphological changes with re‐distribution of pigments from the animal pole resulting in spotted oocytes. The effects induced by Val‐14 p21 rho A were regulated by progesterone in a dose‐dependent manner whereas prior ADP‐ribosylation of the rho protein blocked its activity. About 30 min after microinjection, p21 rho was associated with the plasma membrane. The membrane association of p21 rho and its biological activity were inhibited by lovastatin, an inhibitor of the 3‐hydroxy‐3‐methylglutaryl coenzyme A reductase. The findings suggest that membrane attachment and biological activity of p21 rho depend on isoprenylation of the GTP‐binding protein.